Abstract by Jacob B. Nielsen
Jacob B. Nielsen
Anna V. Nielsen
Chemistry and Biochemistry
Analyzing the Formation of Thrombin-antithrombin Complex using Capillary Electrophoresis
Preterm birth (PTB) is the leading cause of neurological disabilities and infant mortality. Currently, there is no clinical diagnosis for PTB risk, although PTB risk can be assessed via nine biomarkers: six proteins and three peptides. I have been working on developing a method to separate these biomarkers using fluorescence and microchip electrophoresis. One of the biomarkers we need to separate is the thrombin-antithrombin (TAT) complex. However, TAT has to be assembled from thrombin and antithrombin. Under present conditions, this reaction does not go to completion so we see the peaks for the individual components as well as the complex in the electropherogram. To improve the formation of TAT, I have been testing different conditions and quantifying the results using capillary electrophoresis. Elimination of the individual component peaks will decrease the number of contamination peaks in our total biomarker electropherogram.