Abstract by Ellen Parker
Chemistry and Biochemistry
Affinity Monoliths in 3D Printed Microfluidic Devices for the Analysis of Preterm Birth Biomarkers
Preterm birth (PTB) affects 1 in 9 children born in the United States. In recent studies by Graves and collaborators, three peptides and six proteins have been discovered as biomarkers of PTB. My research focuses on developing a 3D printed microfluidic device for quick and inexpensive analysis of PTB biomarkers. I am developing affinity monoliths, which are porous polymers with bound antibodies that are used to extract a target analyte from solution. I am forming affinity monoliths in 3D printed microfluidic devices. The ability of the affinity monolith to retain and elute labeled ferritin is evaluated in order to determine the best conditions for extracting ferritin from clinical samples. By developing a method to extract ferritin from solution, further work can be done to develop a way to extract all the biomarkers so that they can be analyzed and used to predict PTB.