Abstract by Brian Peine
Chemistry and Biochemistry
Labeling Efficiency in Rapid Bacterial Detection
Currently, the most widely practiced approaches for diagnosis of bacterial bloodstream infections require the collection of a large volume of patient’s blood and ample time to process blood cultures to grow sufficient pathogens for detection. These methods generally take one or more days to provide results. There is a current absence of a successful and reliable rapid diagnostic procedure using microfluidic technology, which could expedite the process to under an hour while also limiting the spread of antibiotic resistance. The long-term goal is to create a microfluidic cartridge for detection of specific pathogens such as E Coli and KPC in blood in under an hour. The primary short-term objective has been identification of appropriate conditions (buffers, ionic concentration, procedures, etc.) for capture and elution of a model KPC 90mer DNA sequence with an emphasis on labeling efficiency. Oligreen reagent has presented various advantages which have been optimized to allow for high fluorescene and efficient binding and elution.