Abstract by Michael Beauchamp
Chemistry and Biochemistry
Microchip electrophoresis separation of pre-term birth biomarkers in a 3D printed device
Three dimensional (3D) printing has proven to be a valuable fabrication and prototyping technique for many industries looking to use rapid feedback to drive innovation. Microfluidic devices are an advantageous tool for diagnosing health issues because they use little sample, are portable, cost little to make, and can be automated. Studies of polymerization depth for various exposure times allowed us to formulate our own custom resin for 3D printing channels sufficiently small (<100 μm) for microchip capillary electrophoresis. The analytes we have focused on are ones recently identified to predict potential preterm birth in pregnancies. We were able to separate fluorescently labeled amino acids, peptides, and proteins. Limits of detection, resolution and separation efficiency were determined for the analytes.