Abstract by Anna V Nielsen
Anna V Nielsen
Jacob B Nielsen
Chemistry and Biochemistry
Microchip Electrophoresis of Preterm Birth Biomarkers
Preterm birth (PTB) is the cause of over one million infant deaths and is also responsible for over $26 billion in medical expenses each year. However, no current clinical assessment exists which can predict PTB risk until it is too late to act. Esplin, et al.,1 found that detection of a panel of biomarkers in blood serum can predict PTB risk with 87% selectivity and 81% specificity. We are developing a point-of-care analysis microchip which will allow for early diagnosis of PTB via the combined detection of these nine biomarkers. A key part of this analysis system involves electrophoretic separation and subsequent laser-induced fluorescence detection of these biomarkers. I have developed conditions for separation of seven of the nine biomarkers with acceptable resolution. Experiments are currently being performed to improve this resolution.
1. Esplin, M.S.; Merrell, K.; Goldenberg, R.; et al. Am. J. Obstet. Gynecol. 2011, 204, 391.e1-8.