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Abstract by Jiuzhi Gao

Personal Infomation


Presenter's Name

Jiuzhi Gao

Degree Level

Doctorate

Co-Authors

Ames Ettinger

Abstract Infomation


Department

Chemistry and Biochemistry

Faculty Advisor

Daniel Austin

Title

Opposite polarity electrical charge on electrosprayed bacterial spores: implications for electrospray mechanism

Abstract

Electrospray ionization (ESI) is a commonly used soft ionization technique in mass spectrometry. Maze et al. observed a phenomenon of bipolar fission in which about 1% of the droplets in a positive electrospray were measured to have a negative charge. We have previously demonstrated that ESI can produce desolvated, de-agglomerated, bacterial spores with either positive or negative charges on their surface. However, under some conditions, we observe as many as 23.7% of electrosprayed spores carry a charge with polarity opposite to the ESI tip.  This effect is present for both positive spray and negative spray.  An asymmetric 2-stage image charge detector eliminates ambiguity of charge measurement.  ESI experiments with amino-terminated polystyrene beads of similar size showed no such characteristics.